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An investigation of the oxidatively damaged transcriptome in the human neuronal cells
Author(s) -
Kharel Prakash,
Gadepalli Venkat,
Chattopadhyay Anshuman,
McDonough Jennifer,
Basu Soumitra
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.612.11
Subject(s) - neurodegeneration , oxidative stress , reactive oxygen species , dna damage , microbiology and biotechnology , rna , messenger rna , biology , superoxide dismutase , transcriptome , gene expression , chemistry , biochemistry , dna , gene , disease , medicine , pathology
Reactive oxygen species (ROS) are produced intracellularly and can cause oxidative damage to the biomolecules. Under normal physiological conditions ROS are used in cellular homeostasis and cell signaling but during stress condition they are often overproduced. The overproduction of the ROS is associated with age and neurological disorders, such as, Alzheimer's disease, Parkinson's disease and multiple sclerosis. The weakened antioxidant defense mechanism in the brain of the patients with the neurological disorders makes the neurons susceptible to oxidative damage. The ROS can damage every major class of biomolecules including DNA, RNA and proteins. In the past the focus was mainly on studies of DNA and protein damages, while the detrimental effects of RNA damages only began to be highlighted in recent years. 8‐Hydroxyguanosine (8‐OHG) is the most common oxidative modification found in the RNA. By identifying the modified mRNA molecules during oxidative stress in the neuronal cells (SH‐SY5Y), we aim to delineate the relationships between specific mRNA oxidation and neurodegeneration. We have isolated the modified mRNA from the stressed SH‐SY5Y cells by immunoprecipitation with anti 8‐OHG antibody. Using RT‐qPCR assay we have shown the differential mRNA expression during the oxidative stress, for example, high expression of superoxide dismutase 1 and carbonyl reductase mRNAs. Additionally, we have identified the selectively modified mRNA molecules by using iLumina® RNA sequencing platform. This study will shed light on the yet to be understood link between mRNA oxidation and neurodegeneration. Support or Funding Information No travel support yet.