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The Antimicrobial Property of the Acetone Extract of Cola Acuminate
Author(s) -
Thomas Brianna J,
Telles Caroline M
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.609.15
Subject(s) - antimicrobial , staphylococcus aureus , acetone , traditional medicine , bacteria , chemistry , minimum inhibitory concentration , microbiology and biotechnology , food science , biology , biochemistry , medicine , genetics
The increase in drug‐resistant bacteria during the recent years has sparked an interest in antimicrobial properties of medicinal plants. Thus, the mechanism by which medicinal plants kill bacteria is certainly an important topic that merits further research. Cola acuminate , also known as Obi or Bizzy nut to the Ettu people of Jamaica, is a “cure‐all” herbal medicine. Cola acuminate (Bizzy Nut), possesses a variety of bioactive compounds which exhibit important inhibitory activities against the growth of certain mammalian cell lines, bacteria and fungi. However, the specific chemicals responsible for this bioactivity have not been identified. Thus, we hypothesized that the solid‐liquid extraction of Bizzy Nut, followed by antimicrobial screening would lead to the identification of active antimicrobial agents present in Bizzy Nut. As an initial step in testing our hypothesis, finely ground samples of Bizzy nut were sequentially extracted in a Soxhlet apparatus using 100% hexane, ether, acetone, methane, or water to produce five independent extracts of unique polarity. The antimicrobial activity of the acetone extract was carried out using Staphylococcus aureus and Pseudomonas aeruginosa as test organisms. Our results show that the acetone extract (Biz‐3) possesses antimicrobial activity in Staphylococcus aureus at concentrations as low as 40ppm. There was a dose‐dependent increase in the zone of inhibition of Biz‐3 from 8.5±0.30mm at 40ppm to 11.7±0.1 mm at 120ppm. To purify this antimicrobial activity, we have used SPE to generate three unique fractions of the acetone extract. The antimicrobial screening (Minimum Inhibitory Concentration, MIC) of each of the SPE fraction will be discussed. Our conclusion from this work is that the acetone extract of Cola acuminate (Bizzy Nut), contains putative antimicrobial bioactivity compounds that are effective again gram positive bacteria.