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CD47 is Required for Activation and Clustering of the TCR/CD3/Intraflagellar Transport Complex to the Immune Synapse
Author(s) -
Nugooru Aishwarya,
Kaur Sukhbir,
Singh Satya P.,
Roberts David D.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.601.5
Subject(s) - jurkat cells , immunological synapse , microbiology and biotechnology , cd47 , t cell , biology , cd3 , immune system , t cell receptor , chemistry , immunology , cd8 , phagocytosis
Intraflagellar Transport Protein 57 (IFT 57) and Intraflagellar Transport Protein 20 (IFT 20) have been recently shown as important components of the immune synapse and are required for CD3 clustering on the surface and T cell activation. Using The Cancer Genome Atlas (TCGA) data analysis, we found that CD47 positively co‐related with expression of IFT57 in several cancer types. CD47 is also a regulatory molecule for T cell activation. We hypothesized that IFT57 may play role in activation by a CD47 dependent mechanism. To elucidate, we over‐expressed IFT57‐Flag using Jurkat (Wild‐Type) and CD47 deficient (JinB8) Jurkat T cell lines. We have confirmed that IFT57 expression is positively co‐related with CD47 in Jurkat (Wild‐Type) and JinB8 T cells. We have further found that the up‐regulation of IFT57 leads to an increase in Tumor Necrosis Factor Alpha (TNFα) and downregulates Signal‐Regulatory Protein Gamma (SRP‐γ) mRNA expression. Our preliminary results indicate that cell surface localization of CD3 differs between Jurkat (Wild‐Type) and JinB8 T cells. Currently, we are working on the localization of CD3 and IFT57 during T cell activation and localization to the Immune Synapse. Future studies will examine differential regulation of SIRP‐α, SIRP‐β1 and SIRP‐γ in relation to IFT57 and CD47 using T cells and Raji cells.