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Sequence Analysis of Mutant Chick Embryo DNA
Author(s) -
Vo Jessica,
Byk Jennifer,
Smith Sheila R.,
Benore Marilee Ann
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.594.6
Subject(s) - riboflavin , embryo , mutant , dna , biology , dna sequencing , sequence analysis , sequence (biology) , incubation , genetics , microbiology and biotechnology , gene , biochemistry
Riboflavin Binding Protein is a transport glyco‐phospho‐protein found in all oviparous species, required for transport of the vitamin riboflavin needed for proper embryonic development. A mutant strain of chickens cannot synthesize the protein; the embryos lacking riboflavin die after the thirteenth day of incubation. DNA was extracted from the embryos. Several kits were utilized and compared to determine the best method to isolate pure DNA in large yield. Sequence comparisons to wild‐type white leghorn chickens are used to confirm the DNA sequence causing the mutation. Support or Funding Information University of Michigan Dearborn Campus Grants