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INO80 Chromatin Remodeling Connects Metabolic Gene Expression to Cell Division
Author(s) -
Morrison Ashby J,
Gowans Graeme,
Schep Alicia,
King Devin,
Greenleaf William
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.593.14
Subject(s) - chromatin , regulator , microbiology and biotechnology , cell division , biology , chromatin remodeling , regulation of gene expression , saccharomyces cerevisiae , gene expression , gene , cell , genetics
The coordination of cellular function with the environment is essential for adaptation and survival. S. cerevisiae has a remarkable ability to sense diverse (i.e. nutrient‐rich or □limiting) environments and reprogram their energy metabolism and proliferative capacity accordingly. These adaptive cellular responses are often achieved by rapid inducible changes in metabolic gene expression facilitated by chromatin modification. Previously we identified the INO80 chromatin remodeler as a primary regulator of gene expression in energy metabolism pathways (Yao et al, MCB 2016). We then sought to examine the role of INO80 in coordinating metabolic homeostasis with cell division in metabolically synchronized yeast. Synchronization is achieved through nutrient depletion of yeast cultures followed by constant perfusion of glucose. Subsequently, the Yeast Metabolic Cycle (YMC) is induced, characterized by continuous respiration cycles of oxygen consumption. Within the YMC, most genes are periodically expressed in specific phases of quiescence, cell growth, and cell division. However, little is known about how these genes are so precisely regulated. We find that the INO80 complex is critical for organizing global gene expression and chromatin organization in the YMC. Resulting cellular phenotypes include altered respiration cycles and loss of metabolic coordination with cell division. Thus, the INO80 chromatin‐remodeler is central regulator of connecting metabolic gene expression to cell division. Support or Funding Information National Institutes of Health; R35 GM119580