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Characterization of the SUN Domain Protein Mps3 Function in Gene Expression
Author(s) -
Antoniacci Lisa,
Lukasak Rebecca
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.593.13
Subject(s) - biology , gene , gene expression , genetics , mutant , histone , heterochromatin , dna , saccharomyces cerevisiae , chromatin , microbiology and biotechnology
The yeast nuclear envelope protein Mps3 functions in several aspects of chromosome metabolism such as sister chromatid cohesion, telomere clustering, and DNA damage repair. In addition, Mps3 physically and functionally interacts with the histone variant Htz1. Htz1 was previously identified as a histone variant that prevents DNA from forming silent heterochromatin as a way to regulate transcription. Because of the involvement of both of these genes in chromosome metabolism, it is hypothesized that they may function in regulating gene expression. In order to assess genome expression wild type yeast, mps3‐3 , mps3‐5 , and an htz1Δ strain were examined for genome wide expression levels in both asynchronously and synchronized growing cells. The data generated was analyzed for both up‐regulation and down‐regulation of gene expression in comparison to the wild type strain. Identification of expression differences between the wild type and mutant strains would suggest a function for Mps3 and Htz1 in gene expression. Support or Funding Information Shierling Endowed Chair Marywood University