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Genetic and environmental factors that regulate tandem repeat variation in coding regions
Author(s) -
Fuchs Stephen M
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.591.4
Subject(s) - tandem repeat , biology , genetics , budding yeast , trinucleotide repeat expansion , direct repeat , coding region , saccharomyces cerevisiae , computational biology , phenotype , microsatellite , genome , yeast , gene , allele
More than 10% of all proteins harbor a region of repetitive amino acid sequence. Instability within the DNA that codes for these sequences tunes protein function by varying the number of tandem repeats that are expressed and contributes to phenotypic diversity. Based on the repetitive C‐terminal domain of RNA polymerase II subunit Rpb1p, Our lab has developed a powerful reporter system for measuring expansion and contraction of tandem repeats in coding regions (Morrill et al., J. Biol. Chem , 2016). Here we use this system to identify the pathways involved in both repeat expansion and contraction in the budding yeast S. cerevisiae . We also uncovered a direct role between environmental stress and repeat variation and provide links to how TR variation may be contributing to organismal adaptation to stress. Support or Funding Information Army Research Office