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Metallothionein‐2A mRNA in Peripheral Blood Mononuclear Cells Reflects Dietary Zinc Intake in Healthy Adults
Author(s) -
Hennigar Stephen R.,
Karl J. Philip,
Kelley Alyssa M.,
Anderson Bradley J.,
Armstrong Nicholes J.,
McClung Holly L.,
Berryman Claire E.,
McClung James P.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.459.3
Subject(s) - metallothionein , zinc , peripheral blood mononuclear cell , medicine , endocrinology , chemistry , gene expression , messenger rna , microbiology and biotechnology , immunology , biology , biochemistry , gene , in vitro , organic chemistry
Zinc is an essential nutrient for humans;however, a sensitive biomarker to assess zinc status has not been identified. Metallothionein(MT)‐1 and –2 are ubiquitously expressed proteins that bind cellular zinc with high affinity. MT expression is induced through binding of zinc to metal‐regulatory transcription factor 1, which then binds to metal‐responsive elements in the promoter of the metallothionein gene. The objective of this study was to determine whether expression of MT1 and/or MT2 in peripheral blood mononuclear cells (PBMCs) is associated with dietary zinc intake in healthy human adults. Forty‐three male and female volunteers (33.6 ± 14.3 y, BMI 25.6 ±2.86 kg/m 2 ) completed a Block Food Frequency Questionnaire (v. 2014)to assess habitual dietary zinc intake. Blood was collected and plasma and PBMCs were isolated. RNA was isolated from PBMCs and expression of MT1A and MT2A mRNA was determined by RT‐qPCR. Plasma MT2A protein was determined by ELISA and plasma zinc concentration was determined by atomic absorption spectroscopy. Average daily zinc intake was 15.5 ± 7.44 mg/d (range: 4.89‐31.7mg/d). Plasma zinc concentration (10.8 ± 1.01 μmol/L) did not reflect dietaryzinc intake (r=0.0890; P=0.586). PBMC MT2A expression was 9‐fold greater than MT1A (11.5E‐3± 0.0127, 1.30E‐3 ± 0.00527; P<0.05). MT1A expression in PBMCs was not correlated with dietary zinc intake (r=0.137; P=0.383). MT2A expression in PBMCs was positively correlated with dietary zinc intake (r= 0.302; P<0.05) and increased with increasing levels of dietary zinc (<11 mg zinc/d, 7.29E–3 ± 0.00881; 11–20mg zinc/d, 9.93E–3 ± 0.0141; >20 mg zinc/d, 15.4E–3 ± 0.0114; P<0.05). Plasma MT2A protein was not correlated with dietary zinc intake (r=−0.0840;P=0.605). Findings suggest that MT2A mRNA in PBMCs reflects dietary zinc intake in healthy adults and may be a component in determining zinc status. Support or Funding Information The views expressed in this abstract are those of the authors and do not reflect the official policy of the Department of Army, Department of Defense, or the U.S. Government. Support provided by USAMRMC.