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Lycopene protects kidney against atrazine‐induced oxidative stress and autophagy via activating the AMPK pathway
Author(s) -
Lin Jia,
Xia Jun,
Li XueNan,
Zhang Cong,
Li Wei,
Li JinLong
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.170.6
Subject(s) - oxidative stress , chemistry , superoxide dismutase , catalase , antioxidant , malondialdehyde , biochemistry , pharmacology , keap1 , autophagy , glutathione peroxidase , medicine , apoptosis , transcription factor , gene
The environmental persistence and bioaccumulation of herbicide atrazine (ATR) may pose a significant threat to human health, especially causing nephritic dysfunction. Lycopene (LYC) has been shown to exhibit nephritic disease preventive effects. However, chemopreventive potential of LYC against ATR‐induced nephrotoxicity remains unclear. To determine the effects of ATR and/or LYC on kidney, mice were treated with ATR (50 mg/kg or 200 mg/kg) and/or LYC (5 mg/kg) by intragastric administration for 21 days. The results revealed that ATR led to increased serum creatinine content and significantly histological alterations. Notably, supplementary LYC significantly decreased ATR and its main metabolites (DACT) contents. LYC administration reverted ATR‐induced dysregulation of oxidative stress markers including pancreatic malondialdehyde, hydrogen peroxide and redox enzymes superoxide dismutase, glutathione peroxidase and catalase. LYC treatment downregulated ATR‐induced increase of nuclear factor erythroid 2‐related factor 2 (Nrf2) expression and Nrf2‐regulated redox genes including quinoneoxidoreductase‐1, heme oxidase‐1. In addition, LYC suppressed ATR‐induced activation of autophagy containing LC3II/LC3I, ATGs, Belin1, p62, in parallel with increased AMPK activation. Together, our findings demonstrate that LYC suppresses the expression of antioxidant enzymes and p62 by activating Nrf2 signaling pathway, then stimulating AMPK pathway to resist ATR‐induced renal oxidative stress and autophagy. Support or Funding Information China New Century Excellent Talents in University (No. NECT‐1207‐02), National Natural Science Foundation of China (No. 31572586) and Academic Backbone Project of Northeast Agricultural University (No. 15XG16).