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Eicosapentaenoic acid (EPA) promotes differentiation of human brown precursor cells into metabolically active classical brown adipocytes via Gq/11 signaling
Author(s) -
Fan Rong,
Kim Jiyoung,
Giraud David,
Chung Soonkyu
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.146.6
Subject(s) - gpr120 , eicosapentaenoic acid , adipogenesis , prdm16 , microbiology and biotechnology , g protein coupled receptor , brown trout , receptor , chemistry , agonist , signal transduction , thermogenin , thermogenesis , brown adipose tissue , biology , endocrinology , medicine , biochemistry , fatty acid , adipose tissue , polyunsaturated fatty acid , mesenchymal stem cell , salmo , fishery , fish <actinopterygii>
Eicosapentaenoic acid (EPA) supplementation exerts a plethora of health benefits. Previously, we demonstrated that EPA promotes brown adipogenesis by mediating G‐coupled protein receptor 120 (GPR120) activation in rodent brown precursor cells. The aim of this study is to establish the role of EPA in regulating brown adipogenesis in human classical brown precursor cells. The immortalized human brown precursor cells were employed to induce differentiation in the presence of oleic acid, palmitic acid, and EPA. EPA treatment significantly increased brown‐specific gene expressions including Ucp1, Prdm16, Cidea, DiO2, UCP1 protein expressions and oxygen consumption rate. To further investigate the role of GPR120, a receptor family involved in Gq/11 subtype, human brown precursor cells were treated with either GPR120‐specific agonist cdpA or a potent Gq/11 inhibitor (YM‐254890) during differentiation. GPR120 agonism significantly promoted EPA‐mediated brown adipogenesis, while inhibition of Gq/11 signaling completely abrogated brown adipogenesis. Consistently, the genetic deletion of GRR120 by CRISPR/Cas9 abrogated EPA‐mediated brown adipogenic effects. Collectively, these results demonstrate that EPA supplementation mediates Gq/11 signaling of GPR120 leading to augmentation of brown adipogenesis in human brown precursor cells. Support or Funding Information NIH 1P20GM104320