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Correlations of Abdominal Fat and Colonic Sulfidogenic Bacteria in African Americans and Non‐Hispanic Whites with Newly Diagnosed Colorectal Cancer
Author(s) -
GomezPerez Sandra L,
Yazici Cemal,
Wolf Patricia M,
Braunschweig Carol,
Xicola Rosa,
Llor Xavier,
Ellis Nathan,
TussingHumphreys Lisa,
Mutlu Ece A,
Gaskins Rex H
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.138.7
Subject(s) - waist , medicine , gastroenterology , colorectal cancer , body mass index , cancer
Objective To determine correlations between abdominal fat distribution (AFD) and abundance of gut microbiota bacterial gene targets in African Americans (AAs) and Non‐Hispanic Whites (NHWs) with incident CRC. Methods Adults (n=64; AAs=44, NHWs=20) with incident colorectal cancer with a preoperative abdominal computed tomography scan and non‐involved colonic tissue for assessment of gene targets of sulfate‐reducing bacteria [SRB] in the colonic mucosa were included. A cross‐sectional CT slice at mid‐point of L3 vertebra was analyzed for waist circumference (cm) and surface areas (cm 2 ) of abdominal fat: total (TAT), subcutaneous (SAT), superficial subcutaneous (SSAT), deep subcutaneous (DSAT), and visceral (VAT) adipose tissues. Quantitative PCR was used to measure abundance of SRB gene targets in non‐involved colonic mucosa including: pan‐dissimulatory sulfate reductase A (DSR1F4r), Desulfovibrio (DSV), Desulfobacter (DSB), and Desulfotomaculum (DFM). Pearson correlation stratified by race was used to assess relationships between abdominal fat and gut microbiota. Results Mean age (SD), BMI (SD) and waist circumference (SD) for AA vs. NHW were: 63.4(8.9) vs. 60.2(6.7), 27(5.6) vs 29.2(6.1) and 97.6(11.4) vs 103.9(15.6), respectively. About 60% (27/44) of AAs and 85% (17/20) of NHWs were male. Significant correlations were observed between the abundance gene targets of sulfidogenic bacteria, DSV, DSB, and DFM with cross‐sectional (cm 2 ) levels of SSAT and DSAT. SSAT was negatively associated with the abundance of DSB (r=−0.4985, p=0.0027, n=34) whereas DSAT was negatively associated with abundance of DSV (r=−0.3469, p=0.0262, n=41) and DFM (R=−0.3099, p=0.0486, n=41) in AAs only. No associations for bacterial gene targets and AFD were observed for NHWs. Conclusions These findings suggest that AFD may be related to sulfidogenic bacterial species in colonic mucosa differentially in AAs compared to NHWs. Support or Funding Information Funding: The project described was supported by grant numbers 5R25‐CA057699 and 1R01‐CA204808 from the National Cancer Institute and its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute.