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Behavioral Profile Assessment in Offspring of Swiss Mice Underwent to Neonatal Treatment with Fluoxetine
Author(s) -
Silva Cortes Wellington,
LaureanoMelo Roberto,
Conceição Rodrigo Rodrigues,
Souza Janaina Sena,
Lau Raphael Silva,
Silva Almeida Claudio,
Giannocco Gisele
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1076.11
Subject(s) - offspring , open field , fluoxetine , tph2 , serotonin , serotonin reuptake inhibitor , medicine , endocrinology , tail suspension test , analysis of variance , hippocampus , behavioural despair test , antidepressant , biology , pregnancy , serotonergic , genetics , receptor
Serotonin exerts an important role in the central nervous system embryogenesis of mammals, influencing directly several brain systems ontogeny. Therefore, we investigated the effect of neonatal Fluoxetine (FLU), a selective serotonin reuptake inhibitor, on the behavioral expression of adult male Swiss mice. For this purpose, three groups (n=6 each, and ~35g) of pregnant female Swiss mice were mated. The offspring obtained was treated with FLU (1mg/kg, s.c.) from the 5 th at 15 th post‐natal day. On the 16 th post‐natal day, female puppies were euthanized and hippocampus was dissected for RNA analysis whereas the male offspring, by completing 70 days of life, were underwent a behavioral assessment in open field, light‐dark box and tail suspension test. Statistical analysis was performed by t‐Student and the means were considered significantly different when p<0.05. According to our results, the programmed animals had a decrease in TPH2 (0.07±0.007 vs. 1.07±0.19, p<0.001), 5HT1a (0.08±0.007 vs. 1.02±0.17, p<0.001), SERT (0.35±0.08 vs. 1.09±0.18, p=0.004), BDNF (0.19±0.02 vs. 1.07±0.19, p=0.001) and LMX1B (0.03±0.01 vs. 1.12±0.25, p=0.001) expression. In addition, it was observed less immobility time in tail suspension test (77.6±7.25 vs. 112.1±12.2, p=0.02) and higher grooming time in open field test (7.6±1.4 vs. 3.1±0.9, p=0.01). In light‐dark box test, the animals treated with FLU showed a shorter time in the light side than control (36.69±10.4 vs. 76.2±5.48, p<0.01). These findings suggest that programming with FLU during the neonatal period alters hippocampal serotonergic system, promoting anxiety and depression‐like behaviors in adults. Support or Funding Information FAPERJ & CNPq