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Prenatal Thyroxine Treatment promotes Anxyolisis in Swiss Mice Offspring
Author(s) -
Conceição Rodrigo Rodrigues,
LaureanoMelo Roberto,
Souza Janaina Sena,
Albuquerque Josie Marcelle Marcelle Lira,
Giannocco Gisele,
Olivares Emerson Lopes,
Silva Cortes Wellington
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1076.10
Subject(s) - open field , offspring , fetus , medicine , tail suspension test , hippocampus , pregnancy , endocrinology , thyroid , zoology , physiology , biology , behavioural despair test , antidepressant , genetics
The proper functioning of the maternal thyroid plays a crucial role in fetal development. Thus, the aim of our study was to verify how maternal hyperthyroidism is able to change behavioral parameters in mice's offspring during adulthood. For this purpose, two groups (n=6 each, and ~35g) of pregnant Swiss mice were randomly divided into two groups: control and thyroxin‐treated (T4) group. The control was treated with 0.9% saline, while the treated group received T4 (200μg/kg, s.c. ) during all pregnancy period. By completing 70 days of life, the puppies were underwent to a battery of tests in the following sequence: open field, dark‐light box, elevated plus maze, marble burying, rotarod and tail suspension test. At the end of the experiment, hippocampus was dissected for RNA analysis. Statistical analysis was performed by t‐Student and the means were considered significantly different when p<0.05. According to our results, it was shown that T4 group had an increase of 52.42 % in total distance traveled (16.31± 1.04 m vs . 10.7 ± 0.68 m, p < 0.001) in open field test. It was also shown difference of 13.62% in light time side (126.8 ± 3.10 sec. vs. 111.6 ± 3.59 sec., p = 0.004) in light‐dark box paradigm. In elevated plus maze test, we observed a higher time (42.09 ± 4.29 sec. vs. 24.64 ± 6.00 sec., p = 0.02) and percentage of entries (27.88 ± 3.33 vs. 15.56 ± 2.47, p = 0.008) in the opened arms. In hippocampus, T4 offspring had higher expression of TPH2 (4.26 ± 0.71 vs. 1.33 ± 0.49, p = 0.01), SERT (3.92 ± 0.78 vs. 0.56 ± 0.18, p = 0.005), 5HT1a (1.80 ± 0.18 vs. 1.10 ± 0.19, p = 0.03) and GAD 67 (2.62 ± 0.40 vs. 1.18 ± 0.35, p = 0.02). These findings indicate that gestational hyperthyroidism alters hippocampus serotonergic and gabaergic systems, reducing anxiety‐like behavior in adults. Support or Funding Information FAPERJ & CNPq