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Developmental differences in cardiac transcriptome suggest gene candidates underlying differences in anoxia tolerance in the Western Painted Turtle
Author(s) -
Fanter Cornelia E,
Warren Daniel E
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1075.6
Subject(s) - hatchling , painted turtle , transcriptome , turtle (robot) , biology , acclimatization , andrology , zoology , gene expression , gene , ecology , hatching , genetics , medicine
Adult Western painted turtles, Chrysemys picta bellii, are the most anoxia‐tolerant tetrapod known and is capable of surviving over 170 days of anoxia at 3°C. Hatchling turtles, however can survive only 41 days at 4°C. To investigate the transcriptomic basis for this difference, we compared the anoxia‐induced transcriptional responses in the ventricle of adult and hatchling painted turtles exposed to four different conditions: 20 °C acclimation, five weeks at 3 °C, 20 days of anoxia at 3 °C, and re‐exposure to oxygen at 3 °C. At each time point, adult and hatchling turtles were sampled and RNA extractions were performed on the frozen, homogenized ventricular tissue. Illumina HiSeq 4000 was used to sequence mRNA, and transcript expression levels were compared between treatments to determine the changes attributed to temperature, anoxia exposure and development. Support or Funding Information This work was supported by a CAREER award from the National Science Foundation.