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Ventilatory and Neurochemical Effects of Microdialysis of a μ‐opioid Receptor Agonist (DAMGO) into the hypoglossal nucleus of awake and sleeping goats
Author(s) -
Langer Thomas M,
Neumueller Suzanne,
Burgraff Nicholas James,
Pan Lawrence,
Hodges Matthew R,
Forster Hubert V
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1054.3
Subject(s) - damgo , opioid , microdialysis , anesthesia , rostral ventrolateral medulla , neuroscience , medicine , enkephalin , chemistry , endocrinology , psychology , dopamine , receptor , medulla oblongata , central nervous system
Unilateral dialysis of [D‐Ala2, N‐MePhe4, Gly‐ol]‐enkephalin (DAMGO) into the ventral respiratory column (VRC) of awake and sleeping goats causes a decrease in breathing. This observed decrease is transient, perhaps because of a concomitant local increase in GABA. However, data of others suggest opioids acting on the hypoglossal nucleus (HN) may contribute to opioid induced respiratory depression (ORD) (J Physiol. 2009 Jun 1; 587(Pt 11): 2677–2692). These data suggest neuromodulator compensation may not occur in motor nuclei such as the HN. Accordingly, herein we chronically implanted unilateral microtubules into the HN of adult goats. We then dialyzed DAMGO during both awake and NREM sleep states. We found that dialysis of DAMGO decreased tidal volume and pulmonary ventilation in a majority of goats during both awake and NREM sleep states. In contrast to findings in the VRC, DAMGO dialysis into the HN does not change GABA or any other neuromodulator measured herein in the effluent dialysate. Accordingly, findings to date do not support the concept of neuromodulatory compensation occurring in the HN. Support or Funding Information National Heart, Lung, and Blood Institute grants HL‐25739, HL‐112996, HL‐007852, and by the Department of Veterans Affairs