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CENTRAL TRPV4 AND WARMTH‐DEFENCE RESPONSE IN WISTAR RATS
Author(s) -
Bícego Kenia Cardoso,
Scarpellini Carolina Silveira,
Almeida Maria Camila,
Gargaglioni Luciane Helena
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1040.2
Subject(s) - trpv4 , thermoregulation , microinjection , antagonist , chemistry , endocrinology , medicine , biology , transient receptor potential channel , receptor
Peripheral TRPV4 is activated and triggers warmth‐defense responses at ambient temperatures (T as ) of 26–30°C in non‐genetically modified rats. TRPV4 also can be found in medial preoptic area (MPA), region known by being involved with activation of heat loss mechanisms. Then, the aim of this study was to verify if central TRPV4 in the MPA plays a role in the control of body temperature (T b ) affecting autonomic and behavioral thermoeffectors. To this end, under two different T as (21 e 28°C), the body temperature (T b ) of rats was measured after TRPV4 in MPA were antagonized by HC‐067047. Metabolic rate, heat loss index (HLI) and preferred temperature were also recorded after central blockage of TRPV4. The antagonism of central TRPV4 caused a further increase in the T b of rats (p< 0.001) exposed to 28°C, but not to those ones exposed to 21°C, compared to vehicle‐treated animals. The rise on T b under 28°C was accompanied by an augment in oxygen consumption (p= 0.007) and by reduction of HLI (p=0.041). Lastly, the cold seeking behaviour observed in control animals after exposure to warm T as for 2 hours (28–30°C) was inhibited by the microinjection of the antagonist of TRPV4 into MPA (p= 0.01). Thus, our results indicate that central TRPV4 plays a role in thermoregulation in warm condition, affecting both autonomic and behavioral heat loss responses. Support or Funding Information FAPESP (15/04849‐6)