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Stereological Analysis Of Long‐Term Effect Of Very High Fat Diet To Study The Synergism In Hormonal And Cellular Changes In Male Mice
Author(s) -
Chakraborty Tandra Roy,
Gomez Victoria,
Babu Sathyapriya,
Gurung Devya,
Caraballo Trevor,
Chakraborty Sanjoy
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1037.3
Subject(s) - medicine , endocrinology , leptin , hormone , islet , adipocyte , stereology , insulin , biology , obesity , insulin resistance , caloric intake , food intake , adipose tissue
Objective To determine the hormonal changes that occur as a result of the long term intake of a very high fat diet (VHFD) leads to simultaneous changes in the islets of Langerhans and adipocyte cell size. Methods Male mice were fed with a normal chow diet (ND, n=15) and a VHFD (n=30) for 2, 12 and 24 weeks. Body weight, food intake, caloric intake [fat (saturated and unsaturated), protein and carbohydrate], hormone levels (leptin and insulin), and islet of Langerhans/adipocyte size were quantitatively recorded using stereology. Results In VHFD‐fed animals, body weight showed significant percent increase within the first 12 weeks and then plateaued with time. VHFD‐fed animals consumed significantly less food than ND at all time periods indicating that it was the quality of food and not the quantity that caused this increase in body weight. Stereological analysis of male mice on VHFD showed significant increase in leptin and insulin levels along with accompanying growth in islet and adipocyte size within the first 12 weeks which plateaued as the mice aged. The increases in the islet and adipocyte size in VHFD fed animals were similar to the analogous increases in hormonal levels (2 vs 12 vs 24 weeks). These results, therefore, suggest that in diet‐ induced obesity changes, shifts in hormonal levels works hand in hand with metabolic adjustments at the cellular level to combat the effect of fat. Conclusion Thus, hormonal resistance, changes in adiposity, islet size and caloric intake with prolonged exposure to high fat are probably defensive mechanisms employed to protect against diabetes. In order to understand these complicated and nuanced effects of high fat and to comprehend the underlying mechanism, it is important to focus on long‐term studies that emphasize the synergy between cellular and hormonal changes in addition to an analysis of individual components.