Premium
N‐acetylcysteine Effects on Renal Ischemic Injury Associated to Chronic High Sodium Intake
Author(s) -
Pereira Rafael Canavel,
Romão Carolina,
Gracioli Fabiana,
Shimizu Maria,
Oliveira Ivone,
Furukawa Luzia
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1030.12
Subject(s) - tbars , medicine , endocrinology , renal function , lipid peroxidation , kidney , uric acid , sodium , blood pressure , kidney disease , low sodium , chemistry , oxidative stress , organic chemistry
Introduction Acute episodes of severe renal ischemia result in acute kidney injury. These episodes are followed by a characteristic recovery and repair response, which renal function appear completely restored within one mouth. However, the chronic effects of such an injury associated to high sodium intake and N‐acetylcysteine (NAC) have not been well investigated. Aim To evaluate the NAC effects associated with high sodium diet intake on rats submitted to bilateral ischemia and reperfusion (IR). Material and Methods Eight weeks old male Wistar rats were fed normal sodium diet (1,3% NaCl, 25,0% protein – NSD) or high sodium diet (8% NaCl, 25,0% protein ‐ HSD). Also, at same time, both diet groups received or not NAC (600 mg/L) in the drink water. The animals were submitted or not (SHAM) to bilateral IR for 45 minutes at 10 weeks old. At 22 weeks old, the animals had blood pressure, water intake, urine volume, right kidney and heart masses, plasma renin activity (PRA); serum uric acid, total antioxidant status and thiobarbituric acid reactive species (TBARS) and renal interstitial fibrosis measured. Conclusion The chronic NAC treatment associated to high sodium intake in renal injury, prevented the increase of blood pressure, kidney and heart masses. Also, it decreased lipid peroxidation and renal interstitial fibrosis, showing that it has beneficial effects in rats fed with high sodium diet. Support or Funding Information CAPES and FAPESP Results 1 Variables from male Wistar rats fed normal (NSD) or high (HSD) sodium diet submitted or not (SHAM) to ischemia and reperfusion (IR) and received or not N‐acetylcysteine (NAC) at 22 weeks old.SHAM IR IR+NAC Variable NSDn=8 HSDn=7 NSDn=9 HSDn=7 NSDn=10 HSDn=10Blood‐pressure (mmHg) 147,5±11,2 179,0±10,0 * 144,0±12,3 177,0±11,1 # 157,8±11,0 170,8±9,8 Water‐intake (mL/24h) 30,0±18,5 87,1±19,8 * 30,3±9,0 93,0±45,0 # 31,1±16,7 118,6±46,0 & Urine volume (mL/24h) 13,5±3,7 68,9±17,75 * 25,2±13,3 * 96,0±30,6 # 16,0±7,7 71,5±42,3 & Right kidney mass (g/femur) 0,5±0,05 0,6±0,1 0,5±0,2 0,8±0, 0,5±0,2 0,7±0,2 Heart mass (g/femur) 0,4±0,04 0,4±0,1 0,4±0,03 0,69±0,2 0.4±0,04 0,4±0,1 PRA (ngANG/mL/hr) 14,4±7,7 0,09±0,01 * 5,6±2,9 0,06±0,1 # 9,4±2,0 0,9±1,1 & Uric acid (mg/dL) 1,4±0,2 1,0±0,1 # 1,2±0,3 1,8±0,5 $ 1,5±0,2 2,25±1,4 Total antioxidant status (mmol/L) 1,3±0,1 1,3±0,1 1,3±0,1 1,4±0,03 1,2±0,1 1,3±0,1 TBARS (mmol/L) 2,7±0,65 3,2±0,4 3,7±0,8 3,2±0,7 0,9±0,5 * # 0,6±0,4 & $ Renal interstitial fibroses (%) 0,6±0,2 0,5±0,2 1,8±0,7 * 2,3±1,8 # 1,5±0,6 1,5±0,5Data are expressed as mean ± SD. * p<0.05 vs. Sham‐NSD; # p<0,05 vs. IR‐NSD; & p<0,05 vs. IR+NAC ‐ NSD; $ p<0,05 vs. Sham‐HSD.