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Wnt Is Upregulated Following Skeletal Muscle Injury.
Author(s) -
Keoonela Samantha,
McCormick Skyler,
Carpenter April
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1022.5
Subject(s) - wnt signaling pathway , downregulation and upregulation , lrp5 , skeletal muscle , lrp6 , microbiology and biotechnology , regeneration (biology) , endocrinology , biology , medicine , chemistry , signal transduction , biochemistry , gene
Wnt signaling is implicated in a variety of developmental and regeneration processes. In particular, canonical wnt signaling is upregulated following skeletal muscle injury in mice. There are currently 19 recognized wnt ligands with overlapping function. It is unknown which of the wnt ligands are expressed and the time course of their expression following injury and subsequent repair. To investigate the upregulation of wnt message following skeletal muscle injury, we injected the tibialis anterior of male mice with either saline control, or 1.2% barium chloride. Using qPCR we analyzed the message for wnts days 2,7 and 14 following muscle injury. mRNA for all 19 of the wnt proteins was absent in saline injected muscles at days 2, 7 and 14 days post‐injection. However, day 2 post‐BaCl 2 injection, message for 10 of the 19 wnt ligands (Wnts 2, 2b, 3, 4, 5a, 6, 9, 10b, 11 and 16) was present. Day 7 post‐BaCl 2 injection, upregulation of mRNA for 6 wnt ligands (Wnts 2, 2b, 4, 6, 11 and 16) was maintained. Message for wnt proteins was absent during the late phase of muscle regeneration (14 days post‐BaCl 2 injection). These data suggest that wnt signaling during the early stages of inflammation following tissue injury may be important for the early phases of muscle repair. Studies are ongoing to determine the cellular source of the increased wnt expression and causative studies of mutant mouse models will determine the impact of wnt signaling in muscle repair.