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Does Vehicle Matter on Skeletal Muscle Regeneration Study?
Author(s) -
Srikuea Ratchakrit,
Suhatcho Kanokwan
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1022.3
Subject(s) - myogenin , skeletal muscle , chemistry , regeneration (biology) , myod , dimethyl sulfoxide , medicine , anatomy , microbiology and biotechnology , biology , myogenesis , organic chemistry
Although lipid‐soluble and water‐soluble vehicles were routinely used in research investigation and clinical application, however, whether these vehicles have the effect on skeletal muscle regeneration after intramuscular injection are currently unseeing. Therefore, this study was designed to investigate the effects of intramuscular injections of lipid‐soluble vehicles [olive oil, 40% dimethyl sulfoxide (DMSO), and 40% propylene glycol (PG)] and water‐soluble vehicles [0.9% NaCl, phosphate‐buffered saline (PBS), 0.1% ethanol, and distilled water] on skeletal muscle regenerative capacity. Tibialis anterior muscle of young adult C57BL/6 mice was injured by 1.2% BaCl 2 solution to induce skeletal muscle regeneration. Histological analysis on regenerating muscle fiber morphology was evaluated using hematoxylin and eosin staining. Expression of proteins that regulate skeletal muscle regeneration including skeletal muscle stem cell differentiation (myogenin) and regenerative muscle fiber formation (EbMHC) were investigated using immunohistochemical analysis. The results revealed that regenerating muscle fiber morphology, myogenin positive nuclei, and EbMHC protein expression were not significantly changed in almost tested vehicles compared to no vehicle administration. Nevertheless, presence of degenerative muscle fibers paralleled with increased number of myogenin positive nuclei and EbMHC protein expression were evident after intramuscular injections of 40% DMSO and 40% PG. In contrast, this deteriorate effect did not observe after intramuscular injections of 0.9% NaCl and distilled water which were the solutions that used for 40% DMSO and 40% PG preparation in this study. Moreover, the effects of 40% DMSO and 40% PG was comparable with 2% trypsin (degenerative substance) administration. These results suggest that intramuscular injections of 40% DMSO and 40% PG into regenerating muscle could lead to the recurrent of muscle injury during skeletal muscle regeneration. Hence, the applications of DMSO and PG as vehicle for intramuscular injection could potentially affect the experimental results that obtain during skeletal muscle regeneration study. Support or Funding Information This research project is supported by Mahidol University.