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Quantitative analysis of respiratory control during exercise: Development of a rat model of mimicked exercise under anesthesia
Author(s) -
Miyamoto Tadayoshi,
Kawada Toru,
Ueda Shinya,
Nakahara Hidehiro
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1020.22
Subject(s) - hyperpnea , respiratory system , anesthesia , medicine , control of respiration
Abnormal ventilation during exercise is one of the major manifestations of chronic heart failure. However the lack of good animal models under anesthesia makes it difficult to investigate underlying mechanisms of respiratory abnormality. In this study, we developed an experimental animal model to investigate exercise hyperpnea, and evaluated mimicked exercise‐induced changes in controller and plant elements in normal rats under anesthesia. In anesthetized twenty four rats, we induced muscle contraction by stimulating bi‐lateral distal sciatic nerves at high and low voltage (0.5 and 3.0 V) to mimic exercise. We recorded breath‐by‐breath respiratory gas analysis data, and ran a step protocol while recording respiratory responses. During muscle contraction, the controller curve shifted upward without change in central chemoreflex threshold, to compensate for the plant curve shift accompanying increased metabolism. VE and PETCO2 at the operating point were determined by integrating the controller and plant elements in the respiratory equilibrium diagram. In conclusion, investigation of respiratory regulation during exercise using the novel small animal experimental model will provide an opportunity to clarify the mechanisms of exercise hyperpnea. Support or Funding Information A part of the work including this study was supported by JSPS KAKENHI (Grant Numbers 15H03101)Characteristics of controller, plant subsystems, and equilibrium diagrams at rest and during stimulation of bilateral distal sciatic nerves under anesthesia in normal rats.Characterization of controlling system (controller) in respiratory chemoreflex feedback system.Characterization of controlled system (plant) in respiratory chemoreflex feedback system.