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INVESTIGATING INTERACTIONS BETWEEN POLYCHLORINATED BIPHENYLS, IMMUNE MARKERS AND EXERCISE
Author(s) -
Pillai Mahesh R.,
Keylock K. Todd,
Meserve Lee A.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1020.21
Subject(s) - immune system , inflammation , cytokine , endocrine system , wound healing , medicine , physiology , immunology , endocrinology , hormone
Polychlorinated biphenyls (PCBs) were chemicals produced in large quantities for wide ranging commercial applications. Their production was banned after discovery that their environmental pollution caused disruption of various systems including immunological, neurological, reproductive and endocrine. The immune system plays a vital role in wound healing and disrupting these responses can result in delayed healing. Benefits of exercise include accelerated wound healing and reduced harmful effects of physiological factors like stress and certain environmental toxins. This understanding led to our investigation of harmful effects of PCB on the immune system and exploration of possible moderation of some of those effects by exercise. Concentrations of cytokines involved in the inflammation phase of wound healing were analyzed to study this possible moderation. Interleukin‐6 (IL‐6) and granulocyte macrophage colony stimulating factor (GM‐CSF) concentration was measured in wounds created by punch biopsy in mice (female, C57/Bl6). Concentration of IL‐6 and GM‐CSF in not exercised mice was compared with mice exercised on a treadmill at moderate intensity (approx 70% VO 2 max ) for 30 min/day followed by intraperitoneal injection (in both not exercised and exercised mice) in doses of either 0, 100, 500 or 1000 ppm (wt/wt of mice) of an industrial mixture of PCB congeners (Aroclor 1254). Skin from the wound area was excised and homogenized for analysis of cytokine content in mice that were euthanized at Day 3 or Day 5 (n=3–6). Concentration of IL‐6 in Day 5 mice not exercised was 147% greater in 500 ppm PCB administered mice than mice adminitered no PCB. IL‐6 concentration in Day 5 mice administered 500 ppm PCB was 101% greater in mice not exercised than exercised mice, whereas IL‐6 concentration in Day 3 exercised mice was 61% less in 1000 ppm PCB administered mice than mice administered 100 ppm PCB. GM‐CSF concentration in Day 3 mice administered 100 ppm PCB was 31% greater in exercised mice than in mice not exercised. Concentration of GM‐CSF in Day 5 mice not exercised were 43% and 47% less in mice with 500 ppm and 1000 ppm PCB administration respectively as compared to mice administered no PCB. Wound size measurements were compared to the data obtained from the cytokine markers to help understand how exercise and PCBs affects the wound healing process.