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Molecular mechanism of cytokinesis
Author(s) -
Pollard Thomas D.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.102.4
Subject(s) - cytokinesis , resolution (logic) , live cell imaging , fluorescent protein , fluorescence microscope , biophysics , microscopy , microbiology and biotechnology , fluorescence , biology , fusion protein , computational biology , green fluorescent protein , biological system , chemistry , cell , cell division , computer science , physics , optics , artificial intelligence , biochemistry , recombinant dna , gene
We use three complementary approaches to study the molecular basis of cytokinesis. We characterize the structures and biophysical properties the participating proteins to generate hypotheses about the biochemical reactions. Quantitative fluorescence microscopy of live cells expressing fluorescent fusion proteins gives the numbers of each type of protein in whole yeast cells with one second temporal resolution. High‐speed super resolution localization microscopy (FPALM) allows us to image structures at 35 nm resolution in live cells on a time scale of a few seconds. Comparisons of these quantitative measurements in cells with simulations of molecularly explicit mathematical models of the reactions in our biochemical hypothesis usually suggest ways to improve our hypotheses. Our simulations now reproduce the time course of events during the assembly and constriction of the cytokinetic contractile ring as observed in live cells and predict the outcomes of experimental manipulations. Support or Funding Information NIH research grant GM026132