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INFLUENCE OF DIETARY LARD INTAKE ON THE PROGRESSION OF CARDIOMYOPATHY AND SURVIVAL IN A MURINE DIET INDUCED OBESITY MODEL
Author(s) -
ZemljicHarpf Alice E.,
Nguyen Alexander H.,
Joshi Mughda A.,
Alas Basheer F.,
Schilling Jan M.,
Brenner Sara B.,
Nguyen Uyen P.,
Roth David M.,
Jones Carter W.,
Patel Hemal H.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1014.8
Subject(s) - medicine , overweight , calorie , obesity , endocrinology , heart failure , blood pressure , diabetes mellitus , physiology
Obesity poses an enormous healthcare burden and leads to impaired quality of life. We studied effects of a high fat diet (HFD) on the development of obesity related diseases. We hypothesized that increased dietary lard content will reduce survival in a diet induced Type II diabetes model. Methods Three month‐old C57BL/6J male mice were fed different percentages (%) of kilocalories by fat (4%, 10%, 45%, and 60% lard per kcal) and observed for 31 months, by monitoring 1) food intake 2) body weight, 3) glucose tolerance, 4) cardiac function and remodeling, 5) blood pressure 6) behavior, and 7) survival. Results Three months post diet induction, mice on 45% and 60% HFD were overweight, glucose intolerant, normotensive, and presented cardiac hypertrophy with preserved systolic dysfunction. 12 months post diet induction, balance beam, inverted grid, and open field behavioral tests showed correlation between obesity and reduced voluntary physical activity. At 21 months post, 45% and 60% HFD mice compared to animals on 4% and 10% fat diets were hyposensitive to mechanical stimulation but demonstrated evidence of spontaneous pain, showing preference for gabapentin‐paired environments in the conditioned place preference test. Mice on 45% and 60% HFD developed severe dermatitis, presented weight loss and died prematurely without overt signs of heart failure. Given the average lifespan of male C57BL/6J mice between 24 and 30 months, Kaplan‐Meier survival analysis at 24 months after diet induction revealed a mortality of 100% in 60%‐HFD, 75% in 45%‐HFD, 50% in 10%‐group, and 20% in 4%‐group. Conclusion Although 45% and 60% HFD induced the same degree of obesity and hyperglycemia, mortality rate was significantly higher in mice on 60% HFD when compared to 45% HFD. These observations in obese and diabetic mice show a correlation between increased dietary‐lard content and higher mortality. Support or Funding Information VA Merit Grant; BX001963