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Effects of Cellular Dehydration on Glucose Regulation in Healthy Males ‐ A Pilot Study
Author(s) -
Jansen Lisa T,
Adams JD,
Johnson Evan C,
Kavouras Stavros A
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1014.2
Subject(s) - hypertonic saline , medicine , insulin resistance , endocrinology , insulin , saline , vasopressin , dehydration , homeostasis , tonicity , insulin sensitivity , glycemic , type 2 diabetes , diabetes mellitus , chemistry , biochemistry
Preliminary data from experimental animals have linked dehydration to glucose dysregulation via the action of vasopressin. Also epidemiological data suggest that humans with low water intake or elevated vasopressin are more prone to develop diabetes. PURPOSE Therefore, the purpose of this study was to investigate the effects of cellular dehydration on glucose regulation in healthy males. METHODS Four non‐diabetic males (28.4 y±1, HbA1c 5.6±0.2%, BMI 23.6±1.2kg·m −2 ) were recruited for this preliminary study. Each subject underwent two experimental trials, consisting of a 2 h intravenous infusion of saline (ISO 0.9% and HYP 3.0% of NaCl, 0.1ml·kg·min −1 infusion rate), followed by a 2h oral glucose tolerance test. Blood samples were taken from an antecubital intravenous catheter in 30min intervals. RESULTS Mean plasma osmolality was raised to 300±3mmol/kg for the HYP trial, while ISO maintained at 286± 2mmol/kg. Glycemic and insulin responses post glucose loading seemed to be delayed but spiked during HYP (150.6mg/dl glucose at min 60) while displaying a normal response during ISO (136mg/dl at min 60) trials. Homeostatic model assessment index of insulin resistance (HOMA IR) was HYP 0.93±0.24 vs. ISO: 0.85± 0.59, while Matsuda index of insulin sensitivity was HYP: 12.4±5.9 vs. ISO: 12.7±6.5. HYP seemed to cause increased insulin resistance and decreased insulin sensitivity compared to ISO. CONCLUSION The present data suggest that cellular dehydration induced by hypertonic saline infusion might be linked to glucose dysregulation in normal healthy adults.