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N ω ‐Nitro‐L‐Arginine Methyl Ester (L‐NAME) induced hypertension and cardiorenal oxidative stress: Modulatory effect of the methanolic extract of Azadirachta indica
Author(s) -
Omobowale Temidayo Olutayo,
Oyagbemi Ademola,
Ugbor Fisayo,
Adejumobi Olumuyiwa,
Adedapo Adeolu,
Yakubu Momoh
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1011.10
Subject(s) - oxidative stress , medicine , blood pressure , cardiorenal syndrome , antioxidant , kidney , azadirachta , nitric oxide , endocrinology , pharmacology , chemistry , traditional medicine , biochemistry
Hypertension, a sustained elevation of blood pressure is known to cause both structural and functional abnormalities in both the cardiovascular and renal systems. In this study, we investigated the modulatory effects of the methanol extract of the leaves of Azadirachta indica (AI) on L‐NAME‐induced hypertension and cardiorenal dysfunction via oxidative stress in rats. Fifty rats divided into five (5) groups each containing 10 animals were used in this study. Group A (Control) received only clean tap water for twenty‐one (21) days. Group B was treated orally with L‐NAME alone at the dosage of 40 mg/kg for 21 days. Groups C and D were treated orally with the methanol extract of AI at 100 mg/kg and 200 mg/kg, respectively together with L‐Name for 21 days, while Group E received Enalapril at the dosage of 25 mg/kg alongside L‐NAME for 21 days. Systolic, diastolic and mean arterial pressures were recorded in conscious animals before the termination of the experiment by tail cuff plethysmography. The animals were thereafter fasted overnight, sacrificed and the hearts and kidneys were removed from the animals in each group. Biochemical assays to assess the antioxidant defence system and oxidative stress markers were carried out on cardiac and renal tissues. Treatment with L‐NAME led to a significant (p <0.05) increase in blood pressure and markers of oxidative stress in cardiac and renal tissues. AI caused an improvement in antioxidant defence systems, reduction in markers of oxidative stress and the restoration of blood pressure comparable with that of Enalapril. This study suggests that the methanol extract of AI possesses modulatory functions in L‐NAME induced hypertension and cardiorenal oxidative stress. Support or Funding Information None