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Intracellular and Near‐Membrane Dynamics of L‐type Ca V 1.2 Channel
Author(s) -
Ghosh Debapriya,
NievesCintrón Madeline,
Navedo Manuel F
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.1007.42
Subject(s) - intracellular , fluorescence recovery after photobleaching , microbiology and biotechnology , biophysics , exocytosis , biology , chemistry , membrane , biochemistry
L‐type Ca V 1.2 channels (LTCCs) are involved in myriad of physiological functions ranging from excitation, contraction, transcription and proliferation. Numerous studies had been conducted to understand the electrophysiological and biochemical properties of LTCC. Yet, the intracellular and near‐membrane dynamics of LTCCs are poorly understood. Here, using 4D spinning‐disk‐confocal and near‐membrane Total Internal Reflection Microscopy (TIRFM) recordings, we found that LTCCs exhibit diverse and dynamic intracellular trafficking patterns executed via compartments in form of punctate and larger ovoid vesicles as well as elongated tubules. Total Internal Reflection Fluorescence Recovery After Photobleaching (TIR‐FRAP) showed that both actin and microtubules are equally important in active plasma membrane trafficking of these compartments, where LTCC demonstrate either ‘kiss and run’ or ‘kiss and linger’ type fusion events, while perhaps also supporting distinct patterns of intracellular LTCC movements. These LTCC carrying compartments seems to undergo constitutive homotypic fusion and fission events, near the cell membrane. In conclusion, our study reveals very diverse and unique intracellular and near‐membrane dynamics of LTCCs with profound implications for ion channel trafficking. Support or Funding Information This work is supported by NIH R01HL098200 and R01HL121059.