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Post‐treatment with Ma‐Huang‐Tang ameliorates cold‐warm‐cycles induced rat lung injury
Author(s) -
Han JingYan,
Xiao MengMeng,
Pan ChunShui,
Liu YuYing,
Ma LiQian,
Yan Li,
Wang ChuanShe,
Fan JingYu
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb775
Subject(s) - lung , respiratory system , saline , medicine , pharmacology , chemistry , andrology , pathology
Frequent and drastic ambient temperature variation may cause respiratory diseases such as common cold and pneumonia, the mechanism for which is not fully understood, however, due to lack of appropriate animal model. Ma‐Huang‐Tang (MHT), a traditional Chinese medicine formula, is widely used in China for treatment of respiratory diseases including common cold, upper respiratory tract infection, among others. The present study aimed to investigate the effect of MHT treatment on temperature alternation‐induced rat lung injury and explore the underlying mechanisms. Male Sprague‐Dawley rats (180–200 g) were exposed to a cold environment (−15 °C) for 1 h and then shifted to a warm environment (25 °C) for 30 min. This cold and warm alteration cycled 4 times to imitate ambient temperature variation. Rats were administrated with MHT (1.87 g/kg) or saline by gavage 6 h after cold‐warm‐cycles. Lung injury was assessed 6h and 24h after cold‐warm‐cycles, respectively. Cold‐warm‐cycles induced pulmonary microcirculatory disorders including leukocyte adhesion and infiltration, lung perivascular edema, decrease in the expression of tight junction proteins, increase in the expression and activation of Caveolin‐1, Src and NF‐κB activation, NADPH oxidase subunits p47 phox , p40 phox and p67 phox membrane translocation and inflammatory cytokines production. All the alterations were significantly ameliorated by post‐treatment with MHT. This study showed that rat subjected to cold‐warm‐cycles may be used as an animal model to investigate ambient temperature variation induced lung injury, and suggested MHT as a potential strategy to combat lung injury induced by temperature variation.