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Borderline high blood pressure in people with Miltenberger GP.Mur phenotype is associated with elevated band 3 expression on the erythrocyte membrane
Author(s) -
Hsu Kate,
Lin HuiJu,
Yao ChinChe,
Cheng HanChih,
Kuo MeiShin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb76
Subject(s) - glycophorin , band 3 , red blood cell , blood pressure , erythrocyte membrane , antigen , phenotype , medicine , membrane , biology , endocrinology , chemistry , immunology , biochemistry , gene
The erythrocyte surface antigen GP.Mur (also known as Miltenberger subtype III, or Mi.III) is one of the most clinically important red blood cell (RBC) antigens in the field of transfusion in Southeast Asia. GP.Mur is a hybrid membrane protein based on the structure of glycophorin B, inserted with a piece of glycophorin A. The incidence of GP.Mur is rare in Caucasians and northern Asians, but between 2 – 6% among Southeast Asians. We previously showed that GP.Mur+ RBCs express 10% or more band 3 (Cl − /HCO 3 − exchanger) on the erythrocyte surface. GP.Mur promotes structural and functional expressions of band 3. Our most recent human study uncovered that GP.Mur+ healthy people bear larger capacities of HCO 3 − /CO 2 respiration. Unexpectedly, GP.Mur+ subjects also have slightly higher blood pressure than the control subjects. Objective In this study, we further explored whether borderline hypertension in these healthy GP.Mur+ subjects could be associated with their unusual expression of GP.Mur‐band 3 complexes on the erythrocyte membrane. We quantitated band 3 levels in RBC ghosts, and combined these biochemical data with physiologic measurements from the previous human study. Results The two parameters—blood pressure and band 3 expression, were both higher in GP.Mur+ people, and there were no differences between GP.Mur +/+ and GP.Mur +/− . However, there was a direct correlation between pulse pressure and the concentration of band 3 in GP.Mur +/+ subjects, which was not observed in GP.Mur +/− or the control subjects. By comparison, physiologic HCO 3 − /CO 2 respiration was also slightly faster in GP.Mur +/+ than in GP.Mur +/− subjects. Conclusion From these findings, elevated blood pressure and expedited CO 2 respiration are likely both resulted from GP.Mur‐assisted higher band 3 expression on the RBC membrane. Further, the biochemical and physiologic differences between GP.Mur +/+ and GP.Mur +/− could be due to their variations in the structural organizations of band 3 complexes on the red cell membrane. Support or Funding InformationSupported by MOST 103‐2320‐B‐195‐001‐MY3 from Taiwan Ministry of Science & Technology