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Nucleoprotein‐enriched diet prevents disuse‐induced muscle atrophy in soleus muscle of rats
Author(s) -
Nakanishi Ryosuke,
Hirayama Yusuke,
Ueno Mizuki,
Yoshikawa Madoka,
Maeshige Noriaki,
Fujino Hidemi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb709
Subject(s) - myogenin , myod , medicine , ribosomal protein s6 , endocrinology , biology , skeletal muscle , muscle atrophy , kinase , hindlimb , atrophy , protein kinase a , biochemistry , protein phosphorylation , myogenesis
Disuse leads to a decrease of mass, fiber cross‐sectional area, protein synthesis, and satellite cell activity in skeletal muscle. It is known that the depletion of amino acids reduces muscle protein synthesis. In addition, nucleotides affect cell proliferation and differentiation. Thus, amino acids and nucleotides are essential nutrition for protein synthesis, cell proliferation, and differentiation in skeletal muscle. Therefore, the present study investigated the effect of nucleoprotein, which has combined amino acids and nucleotides, enriched diet during unloading on protein synthesis, activity of satellite cell, and muscle atrophy. Female Wistar rats were assigned randomly into 4 groups: control group (CON), control with nucleoprotein group (CON+NP), hindlimb unloading (HU), and hindlimb unloading with nucleoprotein group (HU+NP). Fiber cross‐sectional area (FCSA) was significantly higher in HU+NP group than HU group although unloading resulted in a decrease of CSA. In addition, the levels of phosphorylated‐ribosomal protein S6 (rpS6) and extracellular signal‐regulated kinase 1/2 (ERK1/2) were significantly higher in HU+NP group than HU group, whereas the levels of phosphorylated‐p70S6 kinase (p70S6K) were equivalent among these groups. Furthermore, the numbers of MyoD and myogenin‐positive nuclei cells were significantly higher in HU+NP group than HU group. These results suggest that nucleoprotein exerts preventive effects on muscle atrophy via activations of rpS6 and satellite cells, which are regulated with ERK signaling independently from p70S6K pathway. Support or Funding Information Supported by Grants‐in‐Aid for Science Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and the internal funds of Nissan Chemical Industries, Ltd.