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Effects of chronic type 1 diabetes mellitus on structural changes, fibrosis, apoptosis and hypertrophy in the rat kidney
Author(s) -
Singh Jaipaul,
Bidasee Keshore R,
Adehgate Ernest,
Howarth Chris,
Iqbal Tahreem
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb651
Subject(s) - medicine , endocrinology , ctgf , fibrosis , kidney , diabetic nephropathy , elastin , diabetes mellitus , muscle hypertrophy , renal function , nephropathy , renal hypertrophy , pathology , receptor , growth factor
Diabetes mellitus (DM) is a major metabolic disorder and if left untreated can lead to diabetic nephropathy (DN). This study investigated the effects of 2 and 4 months of type 1 diabetes on the morphology, fibrosis and apoptosis in kidneys using biochemical and molecular techniques. The results show that DM induced significant (p<0.05; Student's t–test) changes in body weight. DM also increased blood glucose, kidney weight and kidney to body weight ratios, an indication of renal hypertrophy compared to controls. DM also induced structural changes in the kidneys including dilation of tubules, thickened of basement membrane and disorganization of the glomeruli. Interstitial and perivascular fibrosis were also prominent as was the development of apoptosis compared to controls. These changes were accompanied by significant (p<0.05) increases in the gene expressions of collagen 1‐alpha, collagen 3 alpha, fibronectin, elastin, MMP9, TIMP4, CTGF, connexin 43, integrin alpha 5, vimentin TGF‐beta1, BNP and ANP compared to controls. Changes were more pronounced and significantly higher (p<0.05) in older DM rats. The results reveal that DM can be detrimental to the kidney structure and function leading to DN.