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Gene Polymorphism of Matrix Metalloproteinases 3, and 9 in Breast Cancer
Author(s) -
Soliman Naglaa Raafat Abdraboh,
Bayoumi Fatehia Ali
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb65
Subject(s) - breast cancer , single nucleotide polymorphism , allele , oncology , allele frequency , biology , genotype , medicine , malignancy , genetics , cancer , gene
Background Genetic factors have a considerable influence on development and progress of malignancy. Matrix metallo‐proteinases (MMPs) are a group of proteolytic enzymes which are proved to be involved in pathogenesis of breast cancer. Some functional single nucleotide polymorphisms (SNPs) affect the transcriptional activity of genes of MMPs. We aimed in the present study to investigate the possible association of 2 SNPs, C/T polymorphism of MMP9 gene, and 5A/6A polymorphism of MMP3 gene , with the occurrence and clinico‐pathological criteria of breast cancer. Subject and method The present case control study included 59 breast cancer female patients, and 77 tumor free age matched control females. The SNPs were genotyped using polymerase chain reaction ‐ restriction fragment length polymorphism assay. Results Data analysis have shown higher frequency of T allele of C/T MMP9 and A5 of A5/A6 MMP3 in breast cancer patients as compared to control subjects with odd ratio (OR), 95%confidence interval (95% CI) 1.8 (1.02 – 3.26) and 1.2 (0.71 – 1.89) respectively. When the frequency of both alleles was analyzed in comparison to prognostic clinico‐pathological criteria of the disease, there was a significant association of T allele of C/T MMP9 with advanced tumor size and histological grade with OR (95%CI) 2.25 (1.12 – 5.64), 1.8 (0.78 – 4.17) respectively. Conclusion the results of the current study suggested that T allele of C/T MMP9 gene polymorphism is strongly linked to the incidence, growth and differentiation of breast cancer. Support or Funding Information Fund supplied by Dubai Medical College