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In Vitro Invastigation Effects of 4‐hydroxyacetophenone on Rat Torasic Aorta's Vasomotor Activity
Author(s) -
GELEN Volkan,
ÇELEBİ Fikret
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb563
Subject(s) - acacetin , nifedipine , chemistry , aorta , pharmacology , vasomotor , apigenin , vasodilation , traditional medicine , medicine , biochemistry , flavonoid , antioxidant , calcium , organic chemistry
Aim Z. clinopodioides Lam. is also known as a “Field Mint” Seven compounds which have vasodilator activity have been isolated from Z. clinopodioides Lam. and two of them are phenolics compounds and these are acetovanillone, 4‐HAP, four of them are flavonoids and these are acacetin, apigenin, chrysin, thymonin one derivat of cinnamic acid and ethyl 4‐coumarate. In this study, it was aimed to define vasodilator activiy mechanisms of fenolic compound 4‐HAP on isole rat torasic aorta. Material and Method In this study 40 male adult Sprague Dawley type of rat were used. Prepared rings were laid out into the 20 ml organ bath with Kreb solution. Rings were stretched by 1g and they were left to 1 hour incubation period. In the end of the incubation period, FE, KCI, nifedipine, L‐NAME, 4‐HAP, SQ22.536, ODQ, ACh, SKF96365, Propranolol, Atropin, TEA, Gibenclamide, 4‐aminopyridine and U73122 were implemented to bath with a protocol. Results Mechanisms of this relaxed effect was assigned by using antagonists. It was observed that Vasorelaksan effect of 4‐HAP on endothelilal aorta straight muscle contractions which had been inductioned by FE under the existance of L‐NAME was considerably inhibited. Conclusion It was stated that 4‐HAP relaxed FE and KCI contractions and owing to this activity endothelilal tissues on L‐NAME existance notably decreased because of NO networkway. It is firmly believed that as relaxed effect of 4‐HAP declines remarkably under the existance of 4‐aminopyridine and nifedipine on unendothelilal aorta rings, activity could be on K + chanal and L‐type Ca +2 chanal. Support or Funding Information Supported of ÖYP