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Non‐transporter mediated drug permeability across MDCK cell monolayers: Towards an explicit definition of the partition constant for passive drug interactions with cell membranes
Author(s) -
Humphreys Sara
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb547
Subject(s) - membrane , chemistry , biophysics , in vivo , permeability (electromagnetism) , confocal microscopy , monolayer , diffusion , confocal , microbiology and biotechnology , biochemistry , mathematics , biology , physics , geometry , thermodynamics
Drug transport models aim to simulate the passage of a drug through the human body. One transport parameter requiring further refinement is the partition constant for drug partitioning into membranes (Kp). In transport model terminology, Kp is defined as the relative rates of diffusion clearance into (CLi) and out (CLo) of an explicit membrane compartment. Here, we use a scanning laser confocal microscope to perform in vivo kinetics experiments where we monitor the diffusion clearance rates Coumarin 152 as it permeates confluent MDCK cell monolayers grown on Transwell® supports.