Premium
Identification of a new sesquiterpine component from Schisandra chinensis and its anti‐inflammatory effect
Author(s) -
Park Soojin,
Im Dongsoon
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb516
Subject(s) - schisandra chinensis , lipopolysaccharide , nitric oxide synthase , nitric oxide , pharmacology , schisandra , p38 mitogen activated protein kinases , in vivo , chemistry , medicine , mapk/erk pathway , immunology , biochemistry , biology , signal transduction , pathology , traditional chinese medicine , microbiology and biotechnology , alternative medicine
Background In Oriental countries, extracts of Schisandra chinensis have been used as an anti‐fatigue and tonic agent. Because inflammatory and oxidative stress are related to chronic fatigue syndrome, it was hypothesized that Schisandra chinensis has anti‐inflammatory constituents. The effects of new compounds isolated from dry fruits of Schisandra chinensis were studied, to elucidate the anti‐fatigue effect of Schisandra chinesis . METHODS From an extract of Schisandra chinensis fruits, SC‐3162 was isolated. Inductions of inducible nitric oxide synthase and cyclooxygenase 2 by lipopolysaccharide were quantified by Western blotting in peritoneal macrophages isolated from thioglycol‐treated mice. A peritonitis animal model was used to test the in vivo efficacy of SC‐3162. RESULTS SC‐3162 inhibited the inductions of inducible nitric oxide synthase and cyclooxygenase 2 in peritoneal macrophages isolated from thioglycol‐treated mice at the mRNA and protein levels. lipopolysaccharide ‐induced productions of nitric oxide and prostaglandin E 2 were inhibited by SC‐3162. Furthermore, SC‐3162 inhibited the lipopolysaccharide‐induced activations of p38 MAPK, but not that of ERK and JNK in mouse peritoneal macrophages. In addition, SC‐3162 strongly inhibited the accumulation of neutrophils, eosinophils, and basophils in the peritoneal cavity in the lipopolysaccharide‐induced peritonitis model,. CONCLUSION SC‐3162 suppresses inflammatory responses in peritoneal macrophages and could be a potential therapeutic agent for treatment of inflammatory diseases.