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Immunohistochemical Evaluation of Five Antibodies for the Detection of Simian Immunodeficiency Virus
Author(s) -
Machiah Deepa,
Villinger Francois,
Gumber Sanjeev
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb469
Subject(s) - simian immunodeficiency virus , clone (java method) , virology , immunohistochemistry , polyclonal antibodies , biology , antibody , monoclonal antibody , virus , immunology , dna , genetics
Similar to humans with HIV who develop encephalitis, rhesus macaques infected with Simian Immunodeficiency Virus (SIV)are susceptible to develop encephalitis characterized by perivascular accumulation of virus‐laden macrophages and multinucleated giant cells, astrogliosis, microgliosis, and neuronal injury. There are no investigative studies in the literature documenting sensitivity and specificity of available Anti‐SIV antibodies for the detection of viral protein in the tissue samples and therefore, a more formal and comprehensive investigation is warranted to advance our understanding of the disease. In this study, a comparative immunohistochemical analysis was carried out on formalin‐fixed, paraffin embedded sections of lymphoidorgans and brain from SIV infected rhesus macaques ( Macaca mulatta ), experimentally infected with SIVmac251 or SIVmac239. Immunohistochemistry (IHC) was performed using five different antibodies: Anti‐SIV mac251gag mAb (clone FA2), Anti‐SIV mac251 p28 mAb (HRP, clone 3F7), Anti‐SIV mac251 p28 polyclonal antibody, Anti‐SIV mac251 gp130 mAb(clone 5i45) and Anti‐SIV mac251 p17 gag mAb (clone KK59) to determine their efficacy for the detection of SIV viral protein. Immunohistochemical quantitative data are correlated with the viral load to estimate sensitivity of each antibody.