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Effect of Baking on the β‐carotene Content of Orange Flesh Sweetpotato ( Ipomoea batatas ) Puree Bread and OFSP Flour Bread
Author(s) -
Muzhingi Tawanda,
Mbogo Daniel,
Low Jan,
Magnaghi Antonio,
Heck Simon,
Gule Simon
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb433
Subject(s) - food science , carotenoid , carotene , chemistry , orange (colour) , saponification , wheat flour , vitamin a deficiency , beta carotene , vitamin , retinol , biochemistry
Orange Fleshed Sweetpotato (OFSP) is a biofortified crop rich in provitamin A carotenoids, and is being promoted a sustainable food‐based strategy to address vitamin A deficiency in sub Saharan Africa. This study was aimed at evaluating the provitamin A carotenoid retention in bread made using OFSP flour to substitute for 10% of wheat flour, and bread made using OFSP puree to substitute for 45% of wheat flour. Samples of OFSP flour, OFSP puree, bread and ingredients for baking were obtained from local bakers in Kenya. The OFSP flour, OFSP puree, dough and breads were extracted for carotenoid by KOH saponification and hexane extraction. The extracted carotenoids were analyzed in an HPLC system with C30 Carotenoid S column and eluted in methanol, water and methyl tert butyl ether gradient profiles. The major carotenoids of OFSP were all‐trans β‐carotene, and its isomers (9‐cis β‐carotene and 13‐cis β‐carotene). Bread made with OFSP puree had the highest all‐trans β‐carotene content of 3.14 mg/100g dry weigh (dw) compared to 0.08mg/100g dw of OFSP flour bread. Adjusting for moisture content the OFSP puree bread will provide about 507μg all‐trans β‐carotene/slice (39g) of bread. Therefore, two slices of OFSP puree bread will provide 42.25 Retinol Activity Equivalence (RAE), which meets almost 30% of the RDA for vitamin A in children aged 1–3 years old. Support or Funding Information DFID (CIP SUSTAIN) and BMGF (CIP SASHA 2)