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Conditioned Media from Small‐sized Human Umbilical Cord Blood‐Derived Mesenchymal Stem Cells Suppresses a‐MSH Induced Melanogenesis
Author(s) -
Oh Hyun ah,
Kim Ji Hyun,
Kim Ju yeon
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb39
Subject(s) - microphthalmia associated transcription factor , mesenchymal stem cell , umbilical cord , cord blood , stem cell , microbiology and biotechnology , cord lining , chemistry , cellular differentiation , immunology , tyrosinase , biology , adult stem cell , biochemistry , enzyme , gene
Human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSCs) has been extensively investigated in terms of cell therapy. Especially, small‐sized hUCB‐MSCs have multiple biological effects in a variety of diseases. Previously, conditioned media from human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSC‐CM) inhibited melanin synthesis via microphthalmia‐associated transcription factor (MITF) degradation by ERK pathway activation. Base on this, we investigated these melanogenesis activity to vary according to the cell size, type of MSCs. In this study, we show that the effect of conditioned media derived from small sized hUCB‐MSCs (small‐sized hUCB‐MSC‐CM) in melanocytes. Treatment of small‐sized hUCB‐MSC‐CM strongly inhibited the alpha‐melanocyte stimulating hormone (α‐MSH) induced hyperpigmentation in B16F1 cells and Melan‐a cells. On the other hand, conditioned media derived from bone marrow‐MSCs (BM‐MSC‐CM), adipocyte‐MSCs (AD‐MSC‐CM) almost not reduced melanin production. We also found that small‐sized hUCB‐MSC‐CM inhibits tyrosinase activity and down‐regulates melanogenic enzymes in melanocytes. These results small sized hUCB‐MSC‐CM efficiently suppressed melanin synthesis in melanocytes and melanoma cells.