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Lipid‐lowering effects of Zanthoxylum schinifolium Siebold & Zucc. seed oil (ZSO) in hyperlipidemic rats and lipolytic effects in 3T3‐L1 adipocytes
Author(s) -
Kim Tae Woo,
Kim Dae Jung,
Kim Kyoung Kon,
Choe Myeon
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb384
Subject(s) - lipoprotein lipase , lipolysis , endocrinology , medicine , adipose tissue , hyperlipidemia , lipid metabolism , oleic acid , hormone sensitive lipase , chemistry , perilipin , palmitoleic acid , adipose triglyceride lipase , lipid droplet , palmitic acid , biochemistry , fatty acid , biology , diabetes mellitus
Background and objectives A recent study has reported that Zanthoxylum schinifolium Sieb. et Zucc. has anti‐atherosclerotic properties. Therefore, the present study investigates the therapeutic potential of Zanthoxylum schinifolium Sielb. et Zucc. seed oil (ZSO) on blood lipid profile and obesity, which causes atherosclerosis, in Sprague Dawley (SD) rats fed a high‐cholesterol and high‐fat (HCHF) diet. Additionally, the lipolytic effects of ZSO were investigated using 3T3‐L1 adipocytes.. Materials and Methods The composition of ZSO was determined by gas chromatography coupled with mass spectrometry. The effect of ZSO on weight control and blood lipid level improvement in rats fed an HCHF diet was assessed through dietary experiments and molecular biological analysis. Additionally, lipolytic action of ZSO was confirmed by analysis of expression of lipid metabolism‐related biomarkers and measurement of glycerol levels in cultured 3T3‐L1 cells and media. Results ZSO contains oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, and palmitoleic acid. The results show a decrease in total body weight, and liver and epididymal adipose tissue weight, although epididymal adipose tissue weight was not statistically significant. Additionally, rats in the HCHF plus ZSO group showed improved levels of hyperlipidemia indicators. Further, western blot analysis confirmed that the improvement of hyperlipidemia indicator levels was induced by stimulation of lipoprotein lipase (LPL) expression, a consequence of ZSO ingestion. Additional results indicate that at the cellular level, reduction of body weight was owing to phosphorylation of hormone‐sensitive lipase (HSL) by activation of the protein kinase A (PKA) pathway, ultimately leading to lipolysis. Conclusion The current study indicates that the improvement of hyperlipidemia by ZSO in rats fed an HCHF diet is at least, in part, due to the stimulation of cholesterol absorption induced by LPL. Additionally, ZSO‐stimulated lipolysis induced by PKA‐HSL signaling reduced lipid accumulation in 3T3‐L1 adipocytes. Support or Funding Information This work was supported by grants of High Value‐added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs(312001‐03‐01‐HD040), Well‐being Bioproducts Regional Innovation Center project (B0009702) and kangwon national university institute of bioscience & biotechnology (320130015).