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The effect of curcumin in intestinal cells
Author(s) -
Lee Hyuna,
Cho Jin Ah,
Park Eunmi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb375
Subject(s) - curcumin , endoplasmic reticulum , thapsigargin , tight junction , microbiology and biotechnology , xbp1 , apoptosis , cholera toxin , inflammation , chemistry , unfolded protein response , caco 2 , cell , biology , cancer research , pharmacology , immunology , biochemistry , rna , rna splicing , gene
Curcumin, a major component of the Curcuma species, contains antioxidant and anti‐inflammatory properties. Although it was found to induce apoptosis in cancer cells, the functional role of curcumin as well as its molecular mechanism in anti‐inflammatory response, particularly in intestinal cells, has been less investigated. The intestine epithelial barrier is the first barrier and the most important location for the substrate coming from the lumen of the gut. We administered curcumin treatment in the human intestinal epithelial cell lines, T84 and Caco‐2. We examined endoplasmic reticulum (ER) stress response by thapsigargin, qPCR of XBP1 and BiP, electrophysiology by wild‐type cholera toxin in the cells. In this study, we showed that curcumin treatment reduces ER stress and thereby decreases inflammatory response in human intestinal epithelial cells. In addition, curcumin confers protection without damaging the membrane tight junction or actin skeleton change in intestine epithelial cells. Therefore, curcumin treatment protects the gut from bacterial invasion via reduction of ER stress and anti‐inflammatory response in intestinal epithelial cells. Taken together, our data demonstrate the important role of curcumin in protecting the intestine by modulating ER stress and inflammatory response post intoxication. Support or Funding Information National Research of Korea ( 2015R1C1A1A02037579)