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Flavonoid from Conyza bonariensis with cytoprotective activity against H 2 O 2 ‐induced oxidative stress in HepG2 cells
Author(s) -
Cheng XiangRong,
Thabit Riyadh Abdulmajid Saleh,
Sun Jin,
Le GuoWei
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb360
Subject(s) - oxidative stress , flavonoid , chemistry , antioxidant , dpph , sephadex , ethanol , cytotoxicity , rutin , toxicity , glutathione , biochemistry , apoptosis , reactive oxygen species , intracellular , viability assay , in vitro , enzyme , organic chemistry
The objective of this study was the isolation, purification and identification of flavonoid from Conyza bonariensis and the evaluation of its cytoprotective activity against H 2 O 2 ‐induced toxicity in human HepG2 cells. A major flavonoid was isolated using macroporous resin D4020, silica gel and Sephadex LH ‐20 columns from C. bonariensis . The isolate was identified as 5, 7, 2′, 5′‐tetrahydroxy‐3, 4′‐dimethoxyflavone (TDF) by LC‐MS, 1 H NMR and 13 C NMR. The DPPH radical scavenging assay showed that the compound had a generally high antioxidant activity. The highest reducing power of the compound (0.98) was obtained at a concentration of 1.5 mg/mL, with those extracted with ethanol of concentrations between 30–50% having the highest antioxidant activity and yield. The survival of the cells in a dose dependent manner and time, and the treatment of HepG2 cells with 200 μM of H 2 O 2 for 4h resulted in an approximately 36% loss of cellular viability, compared with control cells (P < 0.05). The intracellular ROS production showed relative intensity increased significantly in a time dependent manner after H 2 O 2 treatment compared with untreated cells (100.00 ± 5.24%) (P < 0.05). TDF can ameliorate H 2 O 2 ‐induced oxidative stress and apoptosis through diminishing the MDA generation and ROS level, restoring CAT, GPx, GSH, T‐AOC and SOD activity. These results demonstrated that TDF from C. bonariensis has significant protective effects against H 2 O 2 ‐induced cytotoxicity and oxidative stress in HepG2 cells.