Premium
The thyroid hormone T4 improve antioxidant capability and regulate type II and type III deiodinase activity in MIN6 cell in vitro culture
Author(s) -
Tang Xue,
Wang Fei,
Le Guowei,
Shi Yonghui
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb307
Subject(s) - glutathione , antioxidant , deiodinase , oxidative stress , reactive oxygen species , chemistry , homeostasis , medicine , endocrinology , hormone , secretion , thyroid function , viability assay , cell , biochemistry , biology , thyroid hormones , enzyme
The objective of our study is to analyze whether thyroxine (T4) could maintain the redox homeostasis and insulin secretion function of pancreatic β cells under H 2 O 2 ‐induced oxidative stress. MIN6 cells were treated with/without H 2 O 2 (200μM) for 6h and exposed to T4 (10 −5 M, 10 −6 M and 10 −7 M) for 48 h. Viable cells were harvested, and dichloro‐dihydro‐fluorescein diacetate (DCFH‐DA) was used to detect reactive oxygen species (ROS) level; glutathione (GSH) and glutathione disulphide (GSSG) ratio, total antioxidant capacity (T‐AOC) and malandialdehyde (MDA) level were assayed with the appropriate test kits; cell viability was determined by MTT assay; the activity of type II and type III deiodinase (D2 and D3), conversation rate of T4 to T3 and insulin secretion were also measured in the present study. The results showed that H 2 O 2 induced ROS production and cell death, and induced adverse affects in relation to antioxidant defense systems and insulin secretion. These changes were restored by T4 incubation in a dose‐dependent manner. Moreover, T4 increased the conversation rate of T4 to T3 by regulating D2 and D3 activity. These findings indicates that protective effects of T4 on redox homeostasis and insulin secretion may be related about its role in regulating the antioxidant capacity and D2 and D3 activity. Support or Funding Information The present study was supported by the 12th Five‐Year Plan for for Science and Technology Development (No. 2012BAD33B05)