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Effect of probiotics and Atractylodes/Taraxacum against DSS‐induced colitis in mouse
Author(s) -
Park Min young,
Kwon Oran
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb290
Subject(s) - colitis , goblet cell , atractylodes , medicine , mucin 2 , gastroenterology , mucin , inflammation , inflammatory bowel disease , immunology , biology , pathology , disease , epithelium , biochemistry , traditional chinese medicine , gene expression , alternative medicine , gene
By constituting barrier surfaces, intestinal epithelial cell plays a critical role on maintenance of gut health. Dysregulated immune responses and destruction of cell in intestine causes breakdown in intestinal balance. Dextran sulphate sodium (DSS) is the most widely used chemical for induction of colitis in animal. Thus its treatment induces colonic inflammation, acute diarrhea, and shortening of intestine length with clinical and histological similarity to human ulcerative colitis (UC). Recent researches have shown that probiotics could support gut health by providing good gut microbes and playing beneficial roles in gut. And Atractylodes/Taraxacum was used as oriental medicine for intestinal function. So we hypothesized that probiotics lactobacillus gasseri BNR17 (BNR17) and Atractylodes/Taraxacum could help enhancing gut health through reducing destruction of colon tissue and pro‐inflammatory cytokine increased in DSS treated mouse. Female Balb/c mice (7 weeks old, n =20) were acclimated for 1 week and randomized into 4 groups; normal control group (NC), DSS treated group (positive control, DSS), DSS + BNR17 (DP) and DSS + Atractylodes/Taraxacum treatment at 100 mg/kg B.W. (DA) groups. Materials were treated by gavage for 14 days. 3% of DSS in drinking water was provided and Disease activity index (DAI) was estimated during the last 7 days. Mice were fed chow diet ad libitum. At the end of experimental period, colon length and weight were measured followed by H&E staining to determine the level of mucosal inflammation, cell destruction, and mucin secretion. Body weight, DAI and colon length of DSS group were significantly different with NC group. Colon length was also decreased in DSS induced group. In material administration groups, body weight and DAI tended to increase in DA compared to DSS group. Inflammation and cell destruction of colon tissue observed in histopathology were reduced in DP and DA. Mucin secretion was increased in DA group. These results suggest that probiotics and Atractylodes/Taraxacum has capability of alleviating DSS‐induced colitis by reducing damage and inflammation of colon tissue in mouse. We will examine immunohistochemistry and measure target gene expression relate with immune response, cell proliferation/destruction, and mucin secretion with matching morphological data in further study. Support or Funding Information This work was supported by the Ministry of Science, ICT & Future Planning (Bio‐synergy Research Project NRF2012M3A9C4048761) and BK21 PLUS through the National Research Foundation.