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Dietary supplementation of Walnuts could reduce MPP+ induced neurotoxicity in human neuroblastoma SH‐SY 5Y cells
Author(s) -
Essa Musthafa Mohamed,
AlAdawi Samir,
Manivasagam Thamilarasan,
Guiilemin Gilles J,
Thenmozhi Arokiasamy Justin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb260
Subject(s) - neurotoxicity , neuroprotection , oxidative stress , apoptosis , pharmacology , antioxidant , viability assay , lactate dehydrogenase , chemistry , nitric oxide , biochemistry , mtt assay , medicine , toxicity , enzyme , organic chemistry
Objective Oxidative stress and mitochondrial dysfunction mediated neuronal apoptosis is reported to play a major role in the pathology of Parkinson's disease. Walnuts are able to offer protection to neurodegenerative disease models and rich in natural antioxidants. In the present study, we investigated the protective effects of walnut extract against 1‐methyl‐4‐phenylpyridinium (MPP + ) induced neurotoxicity in SH‐SY‐5Y cell lines. Methods The effect of Walnut extract on MPP + induced cell viability (MTT ‐ 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazoliumbromide assay), membrane damage ‐(lactate dehydrogenase (LDH), oxidative stress (levels of ROS, nitric oxide and GSH and activities of SOD and catalase), mitochondrial membrane potential and apoptosis (activity of caspase 3 and protein expressions of cyt. c, Bax and Bcl‐2) were measured. Results Our results showed that walnuts extract could be able to reduce the neurotoxicity of MPP + and offer neuroprotection in vitro.Conclusion This protective effect of walnuts might be mediated by its potent antioxidant properties. However, further research is necessary to isolate active compounds and performing preclinical and clinical studies to confirm the neuroprotective effects of walnuts in PD. Support or Funding Information The support provided by Internal grant from CAMS, Sultan Qaboos University (IG/AGR/FOOD/14/01) is highly acknowledged.