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Anti‐diabetic effect of Seapolynol™ in 3T3‐L1 adipocytes and C57BL/KsJ‐db/db mice
Author(s) -
Lee YeonJoo,
Choi Jia,
Koh EunJeong,
Hwang JiHyun,
Seo YoungJin,
Song JiHyeon,
Lee BooYong
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb255
Subject(s) - medicine , endocrinology , insulin resistance , glut4 , rosiglitazone , insulin , 3t3 l1 , in vivo , chemistry , adipogenesis , insulin tolerance test , glucose transporter , adipose tissue , biology , insulin sensitivity , microbiology and biotechnology
Seapolynol™ (SN), a complex of marine polyphenol extracts, is produced in brown algae Ecklonia cava. The Brown algae has been demonstrated that it has anti‐oxidant, anti‐bacterial, anti‐inflammatory, and anti‐carcinogenic activity in vitro and in vivo . Also, SN have been reported to possess anti‐hyperlipidemic and anti‐hypercholesterolemia, anti‐obesity and anti‐diabetic activity. But, in type 2 diabetes, the mechanisms of action by SN remain poorly understood. In this study, we investigated the effect of SN on insulin resistance. To test this, fully confluent preadipocytes were induced by MDI with SN (25, 50, 100 μM) for 8 days. Growth medium was changed every 2 days with SN (25, 50, 100 μM) and insulin. On day 8, differentiated cells were harvested and measured using Western blot analysis with specific antibodies. Results were evaluated by ANOVA and Duncan's test (p<0.05). (CON; control, fully differentiated cells, ND; nondifferentiation, preadipocyte, MDI; differentiation cocktail (IBMX (isobutylmethylxanthine) + dexamethasone + insulin). Male db/db mice were divided into four groups receiving SN (60, 150 mg/kgBW/day), rosiglitazone (10 mg/kgBW/day), or vehicle with chow diet for 6 weeks. Consistent with these observations, in 3T3‐L1 and in mice, the expression of insulin signaling such as PI3K and Glut4 was increased in SN supplement group. These results demonstrate that SN improved glucose tolerance and insulin sensitivity in 3T3‐L1 adipocytes and in C57BL/KsJ‐db/db mice.