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Increasing dietary nitrate has no effects on bone loss in ovariectomized rats
Author(s) -
Conley Melissa N.,
Iwaniec Urszula,
Hord Norman
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb251
Subject(s) - ovariectomized rat , bone remodeling , medicine , endocrinology , osteocalcin , osteoporosis , chemistry , bone density , bone mineral , nitrate , estrogen , biochemistry , alkaline phosphatase , enzyme , organic chemistry
Scope Evidence suggests a diet rich in fruits and vegetables reduce bone loss in older adults, although the specific compounds responsible are not known. The objective of the present study was to investigate the capability of dietary nitrate, a component in leafy green vegetables, to reduce bone turnover and loss in an ovariectomized rat model. Methods and results Six‐month‐old, female Sprague Dawley rats either sham‐operated (sham) and fed TD.2018 diet (control) or ovariectomized (OVX) were fed either the control diet with no added nitrate, control diet plus 0.1 mmol nitrate/kg bw/day, or 1.0 mmol nitrate/kg bw/day corresponding to OVX control, low‐ (LDN) and high‐ (HDN) dose nitrate, respectively, for three weeks. Serum bone turnover markers (i.e. collagen type 1 cross‐linked C‐telopeptide (CTX) and osteocalcin), bone mass, bone mineral density, histomorphometric parameters, and bone quality parameters were examined. OVX resulted in increased bone turnover and cancellous bone loss. Three weeks of LDN or HDN supplementation had no beneficial effect on the skeleton of an ovariectomized rat model of bone loss. Conclusions These in vivo data indicate dietary nitrate does not slow bone turnover and loss in an OVX rat model of osteoporosis. Support or Funding Information: This work was funded in part by the OSU Environmental Health Sciences Core Pilot Project Grant Program. M.C. is supported by a NSF Interdisciplinary Graduate Education Research Training Fellowship.