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The Sterilized Fermented Feed Improves Intestinal Barrier Function and Immune Function in Chicken
Author(s) -
Zhang Jun,
Zhu Jianjin,
Sun Jin,
Jiang Rong,
Rahman Md Ramim Tanver
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb247
Subject(s) - tlr2 , ileum , mucin , immune system , lipopolysaccharide , basal (medicine) , barrier function , biology , receptor , tlr4 , medicine , endocrinology , tight junction , immunology , biochemistry , insulin , microbiology and biotechnology
The objective of this study was to evaluate the effects of dietary addition of fermentation metabolites by Sterilized fermented feed(SFF) as a replacement for fermented feed(FF) and antibiotics on expression of genes related intestinal barrier function and immune parameters in chicken. Three hundred and ninety‐six 1‐d‐old broilers with body weight of 41.27 ± 0.49 g were randomly allotted into 4 dietary treatments: 1) Positive control: PC (basal diet + bacitracinzinc80 mg/kg feed + colistin sulfate 20mg/kg feed), 2) Negative control: NC (basal diet), 3) FT (basal diet + 2.0% FF), and 4) SFT (basal diet + 2.0% SFF). After trial, 8chickens from each treatment were randomly selected to collect serum and intestinal samples. Chickens in the PC groups had a decrease (P < 0.05) in serum concentrations of lipopolysaccharide (LPS) compared with the NC, FT and SFT group, and there were no difference among group NC, FT and SFT. In the ileum, supplementation with SFF and FF down‐regulated (P<0.05) the mRNA levels of tumor necrosis factor‐α (TNF‐α) andIL‐6 compared with the NC and PC group. Besides, supplementation with SFF up‐regulated(P<0.05) the mRNA levels of Claudin‐1, Claudin‐3, Claudin‐5, zonula occludens1 (ZO‐1), zonula occludens 2 (ZO‐2), Toll‐like receptor 2 (TLR2), Toll‐like receptor 4 (TLR4), defensins AVBD‐9, mucin 2 (MUC2), secretory IgA (sIgA) and IL‐18 compared with the NC group. Furthermore, the mRNA levels of TLR2, AVBD‐9, Claudin‐3 and Claudin‐5 in group SET were lower (P<0.05) than that of group FT but higher than (P<0.05) group PC. And there were no difference on occluding among all groups. Collectively, the current results indicate that dietary addition of SFF has a beneficial influence on the health condition of chicken by improving intestinal barrier function and immune function. That indicated the probiotics is not the only ingredient that works and the fermentation metabolites should be paid more attention. Support or Funding Information Sanzhi Biotechnology Co. Ltd., Shanghai, China