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Effects of methionine hydroxy analogue on intestinal function and oxidative status in broiler chickens
Author(s) -
Tang Xue,
Zhao Yan,
Le Guowei,
Shi Yonghui,
Sun Jin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb232
Subject(s) - broiler , jejunum , methionine , antioxidant , chemistry , superoxide dismutase , duodenum , catalase , biochemistry , zoology , medicine , food science , biology , amino acid
Methionine (Met), the first limiting factor amino acid, is playing a key role in intestinal function. The present study identified the effects of DL‐methionine (DLM) and its liquid hydroxyl analogue, 2‐hydroxy‐(4‐methylthio) butanoic acid (HMTBA) on growth performance, intestinal digestion and antioxidant capacity in broiler chickens. The changes on antioxidant‐related genes mRNA expression in duodenum and jejunum were also investigated. The results showed that broiler chickens fed with HMTBA exhibited higher body weight gain ( P <0.05) and lower feed:gain as compared to the control group. Adding 0.3% HMTBA to the diet significantly increased apparent digestibility and digestive enzymes activity, and the same result was apparent in catalase (CAT) activity and total antioxidant capacity (T‐AOC), as compared to the control and DLM groups ( P <0.05). Met sources supplementation resulted in a significant decrease in MDA content ( P <0.05) in intestinal tract of broiler chickens. Moreover, HMTBA effectively upregulated the mRNA expression levels of antioxidation‐related genes nuclear respiratory factor‐1 (Nrf‐1), metallothionein‐1 (MT1) and superoxide dismutase (SOD1 and SOD2) in duodenum and jejunum ( P <0.05). The results indicated that better effects of HMTBA on growth performance and intestinal digestion may be realted about its role in regulating the mRNA expression of antioxidant‐related genes. Support or Funding Information The present study was financially supported by Rhodimet Research Grant 2010–2012 1 Performance response to DLM or HMTBA supplementation in broiler chickens at 46 days of ageGroup Body weight gain (g/bird/d) Food intake (g/bird/d) Feed: gain (g:g)Control 67.85±2.26 a 134.85±3.72 1.99±0.01 0.1% DLM 68.18±2.58 ab 130.80±5.23 1.92±0.04 0.3% DLM 70.47±3.37 ab 141.51±0.24 2.01±0.10 0.1% HMTBA 71.81±7.26 ab 136.32±13.26 1.90±0.03 0.3% HMTBA 76.41±0.40 b 142.23±8.46 1.86±0.122 Effects of MET sources on antioxidant levels of intestinal of chickens at 46 days of ageCATU·mgprot‐1 T‐AOCU·mgprot‐1 MDAnmol·mgprot‐1Duodenum Control 3.25±0.27 a 2.34±0.62 8.11±1.02 b0.3% DLM 3.29±0.41 a 2.38±0.78 5.68±0.63 a0.3% HMTBA 3.96±0.28 b 2.25±0.50 4.65±0.54 aJejunum Control 0.91±0.17 a 1.31±0.36 12.8±1.82 b0.3% DLM 0.93±0.11 a 1.41±0.40 15.34±0.77 c0.3% HMTBA 1.13±0.16 ab 1.21±0.23 5.64±0.39 a3 Effects of MET sources on digestive enzymes in duodenum and jejunum of chickens at 46 days of ageAmylase (U/g·min) Lipase (U/g·min) Protease (U/g·min)Duodenum Control 2818.41±268.74 b 96.51±8.51 a 125.57±44.77 a0.3% DLM 1787.96±218.69 a 107.47±10.95 ab 252.51±33.15 b0.3% HMTBA 3092.98±217.91 bc 131.08±11.02 c 214.92±27.48 bJejunum Control 4148.15±151.43 65.63±11.13 a 155.72±11.35 a0.3% DLM 4203.70±173.22 61.14±10.35 a 202.62±22.15 b0.3% HMTBA 4097.88±516.72 98.04±8.84 b 224.17±27.49 b