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Does shark liver oil have a potent antithrombotic effect in spontaneously hypertensive rats?
Author(s) -
Jeon Hyejin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb208
Subject(s) - medicine , prothrombin time , blood pressure , partial thromboplastin time , antithrombotic , endocrinology , thromboxane a2 , platelet , enos , pharmacology , nitric oxide , nitric oxide synthase
The present study was conducted with the aim to investigate the favorable effect of dose‐dependent effect of shark liver oil (SLO) on the improvement of blood circulation by enhancing endothelial function and antithrombotic effects against spontaneously hypertensive rats (SHRs). Rats were randomized into 5 groups (n=10/group): Wistar Kyoto (WKY) rat control; SHR control; SLO1 (45% squalene; 400mg/kg body weight); SLO2 (98% squalene; 400 and 1,020mg/kg). The test materials were administered orally on a daily basis with a normal diet for 8 weeks. 6‐week‐old male SHRs were given test materials by gavage feeding and blood pressure was recorded on a weekly basis. Blood pressure was significantly reduced in SHRs fed the 98% squalene high dose (1,020 mg/kg B.W) group compared to vehicle group (p<0.05). Platelet aggregation induced by thrombin and adenosine diphosphate was significantly reduced in SHRs fed the 98% squalene low dose (400 mg/kg B.W) group compared to vehicle group. However, prothrombin time and activated partial thromboplastin time had no significant differences. Plasma prostaglandin‐I 2 and thromboxane‐B 2 levels were significantly different between WKY and SHR, however, there was no significant different between SHR and SLO‐treated SHRs. These finding suggests that SLO might have a favorable effect for improving blood pressure control and platelet aggregation regardless of dose dependency. In further study, phosphatidylinositol 3‐kinase‐Akt‐eNOS signaling pathway will be observed to elucidate the mechanism of the blood pressure response to SLO. Support or Funding Information * This work was supported by the Ministry of Science, ICT & Future Planning (Bio‐synergy Research Project NRF2012M3A9C4048761) and BK21 PLUS through the National Research Foundation.