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Translocation of Gold Nanoparticles in Model Epithelial Cells (Caco‐2 Monolayers)
Author(s) -
Yao Mingfei,
He Lili,
McClements David Julian,
Xiao Hang
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb201
Subject(s) - cytotoxicity , intestinal epithelium , chemistry , biophysics , nanoparticle , absorption (acoustics) , caco 2 , colloidal gold , monolayer , epithelium , in vitro , biochemistry , nanotechnology , materials science , biology , composite material , genetics
At present, there is limited knowledge about the gastrointestinal fate and potential cytotoxicity of ingested inorganic nanoparticles. In this study, we determined the influence of particle size and concentration of gold nanoparticles (AuNPs) on their intestinal absorption, accumulation and cytotoxicity. AuNPs with different sizes (15, 50, and 100 nm) were subjected to an intestinal epithelium monolayer model to establish their patterns of absorption, accumulation and cytotoxicity. Results from inductively coupled plasma mass spectrometry (ICP‐MS) analysis revealed that as the mean particle diameter of the AuNPs decreased, their rate of absorption by the intestinal epithelium cells increased, but their cellular accumulation in the epithelial cells decreased. Moreover, fluoresce microscopy analysis indicated that accumulation of AuNPs caused cytotoxicity in the intestinal epithelial cells, which was evidenced by depolarization of mitochondria membranes. Our results demonstrated that particle size play an important role in dictating intestinal absorption, accumulation and cytotoxicity of AuNPs. Support or Funding Information USDA (2011‐67021, 2014‐67021)